Online User GuideDMC 2
Next9DMC 2 Camera FeaturesThe Polaroid DMC 2 Digital Microscope Camera captures high-quality digital color images from light microscopes and other opt
99Obtaining Software UpdatesFor the latest information about DMC 2 software updates, visit the Polaroid Web site:www.polaroid.com
Next100DMC 2 Camera SpecificationsSensor2.1 megapixel, 11mm CCDOutput resolutionSuper high resolution (1600x1200 pixels)High resolution (800x600)Norma
101DMC 2 Camera Specifications (Continued)Note: Specifications subject to change without notice.Dimensions2.1x7.2x5.1 in (5.3x18.3x13.0cm)Power supply
102System RequirementsUsing the DMC 2 camera and software to preview, capture and enhance pictures requires a minimum level of computer hardware and s
103PC Minimum System Requirements• 166 MHz Pentium processor and 64 megabytes (MB) of RAM. (96MB of RAM for a TWAIN-compliant image processing program
104Macintosh Minimum System Requirements• PowerPC Macintosh computer or compatible with a SCSI-2 interface and 64 mega-bytes (MB) of RAM. (96MB of RAM
105Installing Software and Setting Up the Camera1 Unpack.2 Install the DMC 2 software.3 Attach the camera to the microscope.4 Connect the camera to th
Next106UnpackingMake sure you have all the following components. Contact your dealer if any is miss-ing or damaged.1DMC 2 camera2power supply and cord
Next107Installing the DMC 2 Software1 Turn the computer on.2 On Windows systems, close any applications that may be running.3 Insert the Polaroid DMC
Next1085 Follow instructions on the screen.6 Remove the CD from the drive.Attaching the Camera to the MicroscopeYou need a 0.63 or 1:1 c-mount adapter
Next10Using the software provided with the camera, you can:• Display a color viewfinder image on your computer screen.• Choose resolution, exposure, s
Next109To attach the camera to the microscope:1 Screw the c-mount adapter into the camera.Note: Finger-tighten the adapter. Do not use tools.2 Insert
Next110Connecting the Camera to the ComputerThe DMC 2 camera requires a SCSI host adapter in your computer. If your computer does not have an adapter,
Next111Setting the Camera SCSI AddressEach device connected to a SCSI host adapter must have a unique address from 0-6. To assure a unique address for
Next112Setting the Camera SCSI Address (Continued)Turn the camera power switch to off (0) and set the SCSI address switch to a value not used by any o
Next113Connecting the SCSI CablesPlace the DMC 2 camera at the end of the SCSI chain as shown or between the com-puter and other SCSI devices. Use the
Next114Setting TerminationThe last device in a SCSI chain must be terminated (or have termination on), while all other devices must be unterminated (o
Next115Connecting the Camera to AC Power and Turning On1 Connect the camera to AC power and turn the power switch on.2 Verify that the green and yello
Next1163 Turn the computer on.4 Wait for the system to install the camera driver and display the normal desktop.
Next117Taking a Test Picture1 Start the DMC 2 program:2 Place a specimen under the microscope and focus it through the microscope eye-piece.3 Click Pr
Next1184 In the DMC 2 main window, choose the appropriate settings for the image being captured:ExposureSensitivityColor Temperature5 Select 8-Bit Col
11The camera has a SCSI connection requiring no frame-grabber or other special hard-ware or software. (The camera requires a SCSI host adapter, usuall
119Camera Limited WarrantyPolaroid Corporation warrants the DMC 2 camera against defects in manufacture or work-manship for a period of one year from
Next120Radio and Television InterferenceFCC Notice (U.S.A.)Note: This equipment has been tested and found to comply with the limits for a Class B digi
Next121FCC Notice (continued)• Connect the equipment into an outlet on a circuit different from that to which the receiver is connected.• Consult the
Next122FCC Notice (continued)This device complies with Part 15 of the FCC Rules. Operation is subject to the follow-ing two considerations: (1) This d
123Notice (Europe)Wa rn i ng : This is a Class A product. In a domestic environment this product may cause radio interference in which case the user m
124Copyright and Trademark Notices© Copyright 2001 Polaroid Corporation, Cambridge, MA 02139 USA.“Polaroid” is a registered trademark Polaroid Corpora
Next125Polaroid Offices and Service CentersAustralia EspañaPolaroid Australia Pty. Ltd. Polaroid (España). S. A.13-15 Lyonpark Road Calle Orense 16 -2
Next126Brasil Hong KongPolaroid do Brasil Ltda. Polaroid (Far East) Ltd.Av. Paulista, 1776/11° andar 32/F Windsor HouseCerqueira Cesar 311 Gloucester
Next127Danmark ItaliaPolaroid a. s. Polaroid (Italia) S. p. A.Blokken 75 Via Piave 113460 Birkerød 21051 Arcisate (Varese)Tlf.: 80011887 Tel.: 0269633
Next128Nederland LuxembourgPolaroid Nederland B. V. Tel.: 08002542Zonnebaan 453606 CH MaarssenP. O. Box 12193600 BE MaarssenTel.: 0204874534New Zealan
12Taking PicturesTypical Picture-Taking Sequence1 Turn on the camera.2 Start the DMC 2 software.3 Frame and focus the preview.4 Choose a capture area5
Next129Norge Schweiz/Suisse/SvizzeraPolaroid (Norge) A/S Polaroid AGIndustriveien 8B, 1473 Skårer Hardturmstrasse 133Postboks 80, 1471 Skårer 8037 Zür
Next130Portugal SverigePolaroid (España) S. A. Polaroid ABSucursal em Portugal Ekholmsvägen 36Edificio Monumental Box 204Avenue Praia da Vitória 71-A,
131U. S. A.Polaroid CorporationTec hnica l Sup portToll free: 1-800-432-5355
132Technical SupportIf you need additional help with your Polaroid equipment, call your nearest Polaroid office at the number listed in Polaroid Offic
133Solving SCSI Communication ProblemsSCSI device not recognizedHorizontal lines, sections missing from imageSystem stops responding during image tran
Next134Problem: Device not recognizedFailure of your computer to recognize a SCSI device could be caused by any of the fol-lowing:Device not turned on
135Device not recognized (continued)Incorrect termination. Check for proper SCSI chain termination. (See instructions for setting termination.)Outdat
136Symptom: Horizontal lines, sections missing from imageIf your images display horizontal lines or bands, check the following:Incorrect termination.
137Symptom: System stops responding during image transferIf your system locks and stops responding to the mouse and keyboard during image transfer, ch
Next138Changing Sync Transfer Rate on Some Adaptec SCSI AdaptersIf your system has an Adaptec 2940 or 3940 SCSI host adapter, it may not recognize a S
13Turning the Camera On1 Turn off the computer.2 Turn the camera power switch to on (1).3 Verify that the green and yellow LEDs are on, indicating AC
1394 Make sure the SCSI Device Settings are as follows:Note: Change these settings only for the SCSI address of the device not recognized. Leave all o
140Installing a SCSI Host AdapterThis device connects to a SCSI host adapter in your computer. Most Macintosh com-puters have a SCSI adapter built in,
Next141IndexAAC power, connecting camera to 115area of interest, choosing 17attaching camera to microscope 108Bblackpoint, adjusting color balance by
Next142Index (Continued)camera, DMC, selecting 15camera, selecting 14camera, turning on 13capture area, choosing 17capturing the image 33Cast found on
Next143Index (Continued)color temperature, setting 26communication problems, SCSI, solving 133copyright notice 124Ddark levels, adjusting manually 49d
Next144Index (Continued)exposure, automatic, calibrating 23exposure, setting automatically 22exposure, setting manually 24FFCC notice 120features, DMC
Next145Index (Continued)Ggamma, adjusting 51grain, reducing in long exposures 56gray point, selecting to adjust color balance 46Grayscale Channel Mixt
Next146Index (Continued)Iimage enhancement 43image size in pixels, selecting 30image type, selecting 29installing software and setting up camera 105in
Next147Index (Continued)Mmaintenance, camera 89marker,calibrated, adding to image 58micron marker, calibrated, adding 58micron marker, calibrated, cre
Next148Index (Continued)Ooffices and service centers, Polaroid 125Output Defaults 80output preferences 79Overwrite Images During Rapid Fire 75Ppackage
14Starting the DMC 2 SoftwareIf more than one DMC camera is connected to the computer, you must select the cam-era to be used in this session. To sele
Next149Index (Continued)Rrapid fire feature, using 40rapid sequence of images, capturing 40Remote Capture Files 78remote preferences 77Reset to defaul
Next150Index (Continued)SCSI host adapter (SCSI card), installing 140sections missing from image, solving 136selecting a DMC 2 camera 15sensitivity, s
Next151Index (Continued)Specular 79starting DMC 2 software 14sync transfer rate, changing on SCSI adapters 138system requirements 102Ttechnical suppor
152Index (Continued)Uupdates, software 99Use existing settings 72Use flash 74Use Remote Capture 77Wwarranty, camera, limited 119whitepoint, selecting
15Selecting a DMC CameraIf more than one DMC camera is connected to the computer, use these steps to select the camera to be used in the current sessi
16Framing and Focusing the Preview1 View the subject through the microscope eyepiece.2 Adjust subject position, focus and lighting according to the mi
Next17Choosing a Capture AreaYou can capture the entire DMC frame or a smaller, selected area.To capture an area smaller than the DMC frame:1 Adjust e
185 Click the Viewfinder Display tab to view only the capture area. The values avail-able in the Image Size box correspond to the capture area you sel
NextContentsNavigating in This Document . . . . . . . . . . . . . . . . . 7DMC 2 Ca
19Choosing Color or Grayscale PreviewYou can view the preview image in the DMC 2 main window in either color or gray-scale. A color preview provides a
20Rotating the ImageTo correct picture orientation, you can rotate the preview image 180 degrees by clicking the rotate button.When you rotate the pre
21Enlarging the PreviewYou can enlarge the view of the preview image for focusing and closely inspecting detail while framing. Enlarging the view does
22Setting Exposure Automatically1 If you have not done so, calibrate automatic exposure for the current lighting and specimen conditions. See Calibrat
23Calibrating Auto Exposure1 Manually set exposure to the optimum position for the current setup. See Setting Exposure Manually.2 Click Calibrate Auto
24Setting Exposure ManuallyDrag the Exposure slider right or left to adjust the camera shutter speed for brighten-ing or darkening the image.Range: 20
25Setting SensitivityChoose a sensitivity (camera gain) setting from the Sensitivity list:• Low (ISO 25 equivalent)• Medium (ISO 50 equivalent)• High
26Selecting Color TemperatureYou can select one of the standard color temperatures to match your lighting:• Tungsten (3200K)• Daylight (5500K)These co
Next27Creating a Custom Color Temperature SettingIf the standard color temperature settings are inappropriate for your lighting, you can create a cust
28Creating a Custom Color Temperature Setting (continued)Macintosh Windows4 Click Calibrate.5 Wait for the Calibrate Color Tempera-ture box to close.6
2Calibrating Auto Exposure . . . . . . . . . . . . . . . . . . . . . . . . 23
29Selecting Image TypeYou can select one of these image types for your final image from the Image Type box:• 48-Bit Color (68.7 billion colors)• 24-Bi
30Selecting Image SizeChoose a final-image size from the Image Size box:• 1600 x 1200 pixels• 800 x 600 pixels• 400 x 300 pixels (Windows systems only
31Image File SizesThe size of the saved TIFF image depends on image type and size, as shown in the fol-lowing table. (File sizes are approximate.)Imag
32Verifying Sharp Focus DigitallyIf necessary, you can fine-tune your image focus digitally with the DMC 2 software:1Click the image to display the zo
33Capturing the ImageAfter you have selected the capture settings in the DMC 2 main window, you are ready to capture the image and transfer it to the
Next34Capturing and Enhancing with DMC 2 SoftwareUse this procedure to capture the image currently displayed in the DMC 2 main win-dow for enhancement
353 Do one of the following:Macintosh Windows• Save the image to disk by clicking Save Image (not available when running the DMC 2 plug-in).• Transfer
36Capturing and Saving Immediately to DiskTo capture the image and save it to disk without further enhancement:1 Click the Capture & Save button.N
37Capturing and Transferring to Another Application (Macintosh)Note: This option is available only if you are running the DMC 2 plug-in within the oth
Next38Capturing and Transferring to Another Application (Windows)Use these steps if you want to transfer the captured image immediately to another ima
3Adjusting Color Balance by Selecting a Blackpoint . . . . . . . . . . . . . . . 47Manually Adjusting Colo
395 Click OK.When you click Capture & Save or the save button in the DMC 2 program, the image appears in the selected application for further enha
Next40Capturing a Rapid SequenceThe DMC camera and software allows you to capture a rapid sequence of images in the camera memory. After the images ar
415 Repeat step 4 as needed to capture and display additional thumbnails until the camera buffer is full.Note: Change the Rapid Fire setting on the Ge
42Printing the ImageTo print the current image from the DMC 2 Enhance window:The image prints at 300 dpi and sized to fill the page.Note: To print at
43Enhancing the Image with DMC 2 SoftwareAfter you capture an image by clicking Capture & Enhance in the DMC 2 main win-dow, the captured image is
44Automatically Adjusting Color BalanceTo have the DMC 2 program analyze the current image and automatically set the color sliders to their optimum po
45Adjusting Color Balance by Selecting a WhitepointTo adjust overall color balance by identifying a whitepoint in the picture, click the white eyedrop
46Adjusting Color Balance by Selecting a Neutral Gray PointTo adjust overall color balance by identifying a neutral gray point in the pic-ture, click
47Adjusting Color Balance by Selecting a BlackpointTo adjust overall color balance by identifying a blackpoint in the picture, click the black eyedrop
48Manually Adjusting Color BalanceDrag the three Color sliders left or right to adjust color balance.The effect of your settings appears in the previe
4Setting DMC 2 Software Preferences. . . . . . . . . . . . . . 71General Preferences . .
49Manually Adjusting Dark LevelsDrag the Dark Levels slider left or right to decrease or increase dark levels.The effect of your settings appears in t
50Manually Adjusting BrightnessDrag the Brightness slider right or left to increase or decrease brightness.The effect of your setting appears in the p
51Adjusting GammaDrag the Gamma slider right or left to increase or decrease gamma (the apparent con-trast between the highlights and the shadows).The
52Viewing a HistogramUse the histogram display to judge the distribution of brightness values in the current image. The horizontal axis represents all
53To view a histogram of brightness levels in the image:1 If the DMC 2 main window is visible, click Histogram on the To o l s menu.If the Enhance wi
54Adjusting SharpnessDrag the Sharpness slider left or right to decrease or increase sharpness.The effect of your setting appears in the preview image
55Viewing the Full ImageIf necessary, you can view the full image for close inspection after capturing but before saving. When viewing the full image,
56Reducing Grain in Long ExposuresYou can reduce image grain in long exposures (longer than 500 milliseconds) by enabling the DMC 2 multi-frame averag
57Adding a LabelThis label appears at the bottom of the image when it is saved, transferred to another application, or printed.Macintosh Windows1 Clic
58Adding a Calibrated Micron MarkerDMC 2 software can add a calibrated scale or micron marker to the lower right corner of your image, providing an ac
5System Requirements. . . . . . . . . . . . . . . . . . . 102PC Minimum Syst
Next59Creating a Calibrated Micron MarkerTo create a calibrated micron marker you can add to the bottom of images for deter-mining dimensions of featu
Next60Creating a Calibrated Micron Marker (continued)Macintosh WindowsThe Micron Marker Calibration win-dow opens and displays the microme-ter image.4
Next61Creating a Calibrated Micron Marker (continued)Macintosh Windows6 Enter or select the units of measure in the Units popup.7 Type a name for the
62Creating a Calibrated Micron Marker (continued)Macintosh Windows(no further steps for Macintosh systems) 8 If you want this calibrated marker to be
63Deleting a Calibrated Micron MarkerIf necessary, you can delete a calibrated micron marker from the list box located below the Add micro marker box:
Next64Saving and Reusing Program SettingsIf you develop settings in the Enhancement window that you expect to use frequently, you can save them to a f
Next65Saving and Reusing Program Settings (continued)To save the current settings in a new settings file:Macintosh Windows1 In the Enhancement window,
Next66Saving and Reusing Program Settings (continued)To load enhancement settings you saved earlier:Macintosh Windows1 In the Enhancement window, clic
67Saving and Reusing Program Settings (continued)To delete a settings file:Macintosh Windows1 In the Enhancement window, click Settings.2 Select the n
Next68Program Settings You Can SaveWhen you save DMC 2 program settings for reuse (see Saving and Reusing Program Settings), the following settings ar
6Polaroid Offices and Service Centers. . . . . . . . . . . . . . 125Technical Support . .
Next69Program Settings You Can Save (Continued)Preferences Box -- General SettingsNote: The state of the Multi-Frame Averaging/Dark Subtract checkbox
70Program Settings You Can Save (Continued)Preferences Box --Remote SettingsPreferences Box -- Output SettingsPreferences Box -- Cameras SettingsNo Ca
71Setting DMC 2 Software PreferencesThe DMC 2 Preferences dialog box (Macintosh) and the Set Preferences dialog box (Windows) let you choose these cat
Next72General PreferencesUse the General tab to select general DMC 2 software operation preferences.On each image capture:Reset to defaults Resets al
Next73General Preferences (Continued)Cast found on auto color balance (available only if Do auto color balance is selected under “On each image captur
Next74General Preferences (Continued)Show preview on startup Expands the Main window to show a preview image when the DMC 2 program starts.Use flash
Next75General Preferences (Continued)Save Viewfinder ROI settings When the DMC 2 program is closed, saves the image capture area (region of interest)
76General Preferences (Continued)Launch External Application Starts the image-editing application specified in the External Application Path box when
Next77Remote PreferencesUse the Remote tab to capture images using the remote trigger. You can specify if you want the captured images transferred to
78Remote Preferences (Continued)Save Directly to a File Captures images and saves them directly to a file for viewing and enhancing at a later date.R
Next7Navigating in This DocumentThis Polaroid electronic document uses the Adobe Portable Document Format (PDF) and its associated Acrobat Reader soft
Next79Output PreferencesUse the Output tab to select the highlight property and the output defaults of the images.Highlight Property:Specular Usually
Next80Output Preferences (Continued)Output Defaults:For best results, choose the appropriate channel mixture and output scale for your specimen and th
Next81Output Preferences (Continued)Color Channel Mixture (continued)User Use the custom color channel mixture in MATRIX2PUSER.MTX (for DMC 2 cameras
Next82Output Preferences (Continued)Grayscale Channel MixtureThe Grayscale Channel Mixture uses the following (RGB channels) to produce a grayscale im
Next83Output Preferences (Continued)Grayscale Channel Mixture (continued)Cyan (DMC Ie cameras only) Provides the effect of a cyan filter.Magenta (DMC
84Output Preferences (Continued)Grayscale Output ScaleTone Scale Optimizes the output image tone values for display on amonitor.Linear Sets the tone
85Camera PreferencesUse the Select Camera tab to select the active DMC camera when multiple DMC cam-eras are connected to the computer.Cameras are ide
Next86Using the Color Stacker UtilityThe Color Stacker utility provided with DMC 2 software provides a way to combine three images. This is useful whe
87Creating a Composite Image (Continued)Macintosh Windows2 Open the Color Stacker utility (dou-ble-click Polaroid Color Stacker).3 Select an image for
88Color Stacker Preview OptionsThe Color Stacker utility displays a preview of the composite image as you select files for each color channel. By clic
8Click to display these help pages.Click to open the index.
89Camera MaintenanceDMC 2 camera maintenance consists of cleaning the camera when necessary.Click on either topic below for more information.Cleaning
Next90Cleaning the Camera OpticsImportant: Protect the DMC 2 camera from dirt, dust, rain and moisture. Damage caused by these conditions may not be c
Next91Cleaning the Camera Optics (Continued)Verifying the Location of Dust or DirtAvoid needless cleaning of the DMC 2 camera optics by determining th
Next92Cleaning the Camera Optics (Continued)3 Follow these steps to determine whether the dust spot is on the camera optics or the c-mount adapter len
Next93Cleaning the Camera Optics (Continued)Camera Optics Cleaning Steps1 If you have not already done so, install the shutter-opening software on you
Next94Cleaning the Camera Optics (Continued)2 Obtain the following cleaning materials:3 Detach the camera from the microscope, and remove the c-mount
Next95Cleaning the Camera Optics (Continued)6 Run the shutter-opening software:7 Power down the computer and turn the camera power off. (The shutter r
Next96Cleaning the Camera Optics (Continued)8 Clean the anti-aliasing filter (behind the shutter) with air. If necessary, gently dis-lodge any dust wi
97Cleaning the Camera Optics (Continued)c If dust or dirt remains after the first attempt to wipe it away, repeat the proce-dure with a new, clean wip
98Cleaning the Camera BodyIf the DMC 2 camera body requires cleaning, use a clean, soft cloth dampened with water.Do not use cleaners of any kind, and
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